Dylight649 fluorescent dyes have stronger fluorescence intensity and higher optical tolerance. Abbkine offers DyLight 649 Red Fluorescent Secondary Antibody Fluorescence for higher specificity and diversity to meet most of your type of immunoassay needs. Look at the following form:
Figure 1: The ortho position between human jejunum is labeled with an anti-PGP primary antibody, and the corresponding DyLight 649 secondary antibody (red, upper left) and Cy5 secondary antibody (red, upper right) secondary antibody. It can be seen that the red fluorescence of the DyLight 649 marker is more dense.
Figure 2. Cytokeratin 18 protein in human lung cancer A549 cells is marked red by anti-Cytokeratin 18 antibody and DyLight 649 anti-rabbit secondary antibody, and blue part is Hoechst negatively stained nucleus
Figure 3. Human peripheral blood lymphocyte maps labeled with anti-CD3 and various fluorescein-conjugated secondary antibodies in flow cytometry. Green is DyLight 649, yellow is APC, and blue is Cy5 labeled secondary antibody. Alexa Fluor 647 is a red area. The arrow indicates that Alexa Fluor 647 has a slight background in this experiment.
The DyLight 649 red fluorescently labeled secondary antibody is brighter than the Cy5 and Alexa 647 markers and has a lower background than the Alexa 647 marker. DyLight 649 and APC-labeled red fluorescent secondary antibodies are the best choice for flow cytometry in the appropriate band. In addition, the DyLight 649-labeled secondary antibody is the best choice for the far-infrared emission spectrum when confocal microscopy is used for multi-label detection. In addition, DyLight fluorescently labeled secondary antibodies are more cost effective than traditional FITC/Cy2 and Alexa 488.
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Contact Amytech Co., Ltd. for product information and complete experimental solutions.Source: DyLight 649 Red Fluorescent Secondary Antibody: http://
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